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. 2021 Nov 19;9:85. doi: 10.1186/s40364-021-00326-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Molecular profiling and clinical application of CTCs. a The difference in the metabolic profile between CTCs and lymphocytes of CRC patients. The analysis of the metabolic profile of CTCs shows the potential for the early diagnosis and differential diagnosis of cancer; b Fish images of different CTC phenotypes; c, d The hot map of genomic alterations in CTC and ctDNA in head and neck cancer patients and CRC patients. The associations between genomic alterations detected in ctDNA and CTC in CRC; e Multivariable analysis of overall survival of mCRC patients treated with regorafenib, by CTC counts at baseline. a Copyright Wiley 2019. Reproduced with permission from reference [93]; b Copyright Springer Nature 2018. Reproduced with permission from reference [100]; c, d Copyright Wiley 2019. Reproduced with permission from reference [115]; e Copyright Wiley 2020. Reproduced with permission from reference [118]