FIG. 7.

Analysis of protein binding to and expression of target genes in S-phase cells. Cross-linked chromatin from synchronized S-phase cells was immunoprecipitated with antibodies against E2F1-5 (A) or pocket proteins (B) and analyzed as described in the Fig. 2 legend. Replicate plates of cells were harvested, and cytoplasmic RNA was prepared from these cells. Expression levels of target genes were measured by RT-PCR analysis of 100 ng of RNA from asynchronous log-phase cells, quiescent serum-starved cells (0 h), or S-phase cells (16 h) (C). IP, immunoprecipitation; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.