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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Gastrointest Endosc. 2021 Jun 29;94(6):1119–1130.e4. doi: 10.1016/j.gie.2021.06.016

Figure 4. Immunohistochemistry shows efficient human Factor IX expression in pigs after hydrodynamic delivery.

Figure 4.

A, IHC for hFIX in human liver tissue demonstrated homogenous, cytoplasmic expression in hepatocytes. Control tissue from a non-injected pig liver demonstrates only light background staining for porcine FIX appreciated. Immunostaining in hFIX-injected pig no. 3 revealed abundant hFIX-hepatocytes, with similar cytoplasmic staining to human hepatocytes with variable intensity (bar = 50 μm). B, An example of a liver section stained for hFIX at low magnification power is presented from pig no. 3, left medial lobe (LML) proximal section, demonstrating that immunostaining can be observed in every single lobule, with intensity highest in the center of the lobule (bar = 1 mm). A magnified image from the same liver biopsy section is also presented, showing intense staining bordering the central vein and radiating to the lobule borders (bar = 200 μm).