FIG. 2.

(a) Water intake and urine output in Aldor1^+/+, Aldor1^+/−, and Aldor1^−/− mice. Water consumption and urine excretion by wild-type (n = 20), heterozygous (n = 15), and knockout (n = 20) male mice during a 24-h period were determined. Mice were housed individually in metabolic cages, and values were determined over a 2-day period. Data are means ± standard errors of the means. ∗, P < 0.0001 compared with Aldor1^+/+ and Aldor1^+/− mice. Statistical significance was determined by the unpaired Student t test. (b) Urine-concentrating ability before and after a 24-h water deprivation period or AVP injection in Aldor1^+/+ and Aldor1^−/− mice, measured as urine osmolality. White bars, before deprivation or AVP injection; dark bars, after AVP injection; striped bars, after deprivation. Data are means ± standard errors of the means (n = 4). ∗, P = 0.0005; ∗∗, P = 0.0024; ∗∗∗, P = 0.0014; ∗∗∗∗, P < 0.0001. Statistical significance was determined by the unpaired Student t test.