Fig. 6.

Var gene expression profiles. Log₂ fold change in var transcript copy number relative to single copies in P. falciparum 3D7 gDNA. P. falciparum 3D7 kahrp– parasites cultured ex vivo from three subjects (kahrp– subject 5, kahrp– subject 6, and kahrp– subject 7) were compared with two replicates of parasites inoculated on day 0 (kahrp– pre inoc.1 and kahrp– pre inoc.2). Parasites were collected from subjects by taking blood samples immediately prior to antimalarial treatment initiation; three replicate samples were collected from subject 7 (kahrp– subject 7.1, kahrp– subject 7.2 and kahrp– subject 7.3). The duration of in vitro culture for each sample is specified in Additional File 3: Table S1. As a control, wild-type P. falciparum 3D7 parasites cultured ex vivo from two subjects (WT subject 1 and WT subject 2) in a controlled human malaria infection study using the induced blood stage malaria model [45] were compared with parasites inoculated on day 0 (WT pre inoc.). Blue bars indicate high expression, red bars indicate low expression, and black bars indicate that no expression was detected. Rows list var gene group and accession numbers