Table 2.

Volume and order of reagent distribution at each step of the TR-FRET assay in a 384-well plate

	Background (“BKG”)	Total binding (“TB”)	Non-specific binding (“NSB”)	Test compounds (“Test”)
Cells	10 μL	10 μL	10 μL	10 μL
TagLite buffer 1×	2 μL	2 μL	–	–
Test compounds (7×)	–	–	–	2 μL
Non-labeled RBD (7×)	–	–	2 μL	–
Seal the plate and incubate at 20°C–22°C for 15 min
TagLite buffer 1×	2 μL	–	–	–
RBD-d2 (7×)	–	2 μL	2 μL	2 μL
Seal the plate and incubate at 20°C–22°C for 2 h
Read the plate in a TR-FRET-compatible plate reader