REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
SARS-CoV-2 spike RBD protein	Sino Biological	Cat# 40592-V08H
SARS-CoV-2 spike S1-S2 protein	Sino Biological	Cat# 40589-V08B1
d2-labelled SARS-CoV-2 spike RBD protein	Cecon et al., 2021	N/A
d2-labelled SARS-CoV-2 spike S1-S2 protein	Cecon et al., 2021	N/A
Terbium cryptate Lumi4-Tb	Cisbio Bioassays	Cat# SSNPTBX
TagLite labelling medium	Cisbio Bioassays	Cat# LABMED
jetPEI transfection reagent	Polyplus-transfection	Cat# 101-10N
Cell Dissociation Solution Non-Enzymatic	Sigma-Aldrich	Cat# C5789
Dulbecco’s modified Eagle’s medium (DMEM)-Glutamax	Life Technologies	Cat# 31966047
TRYPSIN 0.25% EDTA	Life Technologies	Cat# 25300096
Penicillin/streptomycin	Life Technologies	Cat# 15140130
Trypan blue solution	Sigma-Aldrich	Cat# T8154
PlasmoTest kit	InvivoGen	Cat# Rep-pt1
10× phosphate buffered saline (PBS)	Life Technologies	Cat# 14040174
Experimental models: Cell lines
Human: Human Embryonic Kidney (HEK) 293 cells	Sigma-Aldrich	RRID:CVCL_0063
Recombinant DNA
SNAP-ACE2 plasmid	Cecon et al., 2021	Addgene id:178592
Software and algorithms
Image J	Schneider et al., 2012	RRID: SCR_003070 https://imagej.nih.gov/ij/download.html
GraphPad Prism 6	GraphPad Software Inc	RRID: SCR_002798 https://www.graphpad.com/scientific-software/prism/
Other
Cell culture incubator at 37°C, 5% CO2	N/A	N/A
Centrifuge for 1.5 mL microcentrifuge tubes	Eppendorf 5427 R	N/A
Tecan F500	Plate reader	N/A
384-well white Flat Bottom low-volume microplate	PerkinElmer	Cat# 6008280
Plate sealing polyester films	VWR	Cat# 391-1250