Figure 5.

Bleeding phenotype and cell survival of LV-VEC.hBDD-FVIII HA BOECs after implantation in the Cell Pouch device

(A) Bleeding assay on mice transplanted with 10 × 10⁶ or 20 × 10⁶ HA and LV-VEC.hBDD-FVIII BOECs, or left untreated (n = 3–6, mean ± SEM, ∗∗p < 0.05; ns, not significant). NSG mice were used as control for bleeding assay. (B) The transplanted Cell Pouch devices were removed from the recipient NSG-HA mice, and immunofluorescence was performed to detect cell survival within the mouse tissue by human cell staining (HLA-ABC) and blood vessel formation through staining with cross-reacting human/mouse von Willebrand factor (vWF) antibody. The images shown are representative of two transplant groups (10 × 10⁶ n = 5; 20 × 10⁶ n = 12). (C) Quantification of HLA-ABC and blood vessel formation from blinded histopathological assessment.