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. 2021 Nov 8;12:736301. doi: 10.3389/fphar.2021.736301

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Copyright © 2021 Zhan, Deng, Wu, Xu, Wang, Li and Pan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Alkalinephosphatase (ALP) staining and (B) its quantitative analysis of BMSC cells cultured on the scaffolds for 14 days. (C) Alizarin red (AR) staining and (D) its quantitative analysis of BMSC cells cultured on a culture plate, PLLA, PLLA/LC, and HAP-PLLA/LC fiber scaffold for 14 days, respectively. (E) Real-time quantitative PCR (RT-qPCR) analysis of osteogenic and vascularized-related gene expression of cells after culturing for 7 and 14 days.