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. 2021 May 7;76(12):1209–1218. doi: 10.1136/thoraxjnl-2020-215460

Figure 2.

Figure 2

Decreased proportions of circulating NSM B cells in patients with PAH. (A) Representative gating strategy for the identification of B-cell subsets in peripheral blood. Starting with live single cells, total B cells (CD19+), transitional B cells (CD19+CD38+CD27−), plasmablasts (CD19+CD38hiCD27+), naive B cells (CD19+IgD+CD27−), NSM B cells (CD19+IgD+CD27+), IgD− memory B cells (CD19+IgD−CD27+), IgM+ memory B cells (CD19+CD27+IgD−IgM+) and switched memory B cells (CD19+CD27+IgD−IgM−) were identified. (B) Proportions of circulating total B cells and the indicated B-cell subpopulations in HC (n=19) and patients with IPAH (n=13), CTD-PAH (n=9) and CHD-PAH (n=7). The results are shown as median (IQR), p exact values were obtained following Kruskal-Wallis test. Dots represent values in individual patients or HC. CHD-PAH, congenital heart disease pulmonary arterial hypertension; CTD-PAH, connective tissue disease-associated pulmonary arterial hypertension; FCS, forward scatter; HC, healthy controls; IPAH, idiopathic pulmonary arterial hypertension; NSM, non-switched memory.