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. 2021 Nov 8;9:743910. doi: 10.3389/fcell.2021.743910

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Copyright © 2021 Shen, Wang, Qiu, Li, Wang, Xue and Tang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Stemness analysis of 10 hub genes in bulk and single-cell RNA-seq data. (A–C) UMAP plot of 130 single cells using different classifications. (D) Expression levels of three CSC markers in single-cell and pooled-cell samples. (E) The expression levels of the three CSC markers were consistent with the FACS-sorting results. (F) Expression levels of 10 autophagy genes in triple-negative and triple-positive samples. (G) Correlation analysis between autophagy genes and stemness scores in TCGA-LIHC patients. (H) Correlation analysis between 10 autophagy genes and three CSC markers in the ICGC database. (I) Correlation analysis between 10 autophagy genes and three CSC markers in the TCGA database. *P < 0.05 and **P < 0.01.