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Characterization of EGFR/HER2, MAPK and PI3K/AKT/mTOR pathways. Phosphorylation state and total protein levels were determined by Western blotting for MDA-MB-231 (A) and MDA-MB-468 (B) cells cultured on ES PCL scaffolds (3D) compared to corresponding 2D control. GAPDH was used as a control of protein loading. Results shown are representative of those obtained from 3 independent experiments.
