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. 2021 Nov 8;9:779432. doi: 10.3389/fcell.2021.779432

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Copyright © 2021 Dai, Ye, Zhong, Chen, Hong, Zhao, Su and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gsdmd deficiency attenuated inflammatory cytokine secretion, cardiac inflammation, and activation of the NF-κB signaling pathway induced by LPS. (A,B) The levels of Interleukin-1β (IL-1β) and Tumor necrosis factor-α (TNF-α) in serum from each group were detected by ELISA (n = 8). (C,D) The mRNA levels of IL-1β and TNF-α in heart tissue from each group were detected by PCR. The results were normalized to the expression of β-actin (n = 8). (E) Representative CD68 immunohistochemistry images of heart tissue from each group (magnification × 400). Arrow heads indicate positive staining. (F) MPO activity in heart tissue from each group (n = 5). (G,H) Expressions of NF-κB P65 and phosphorylated P65 protein in heart tissue were measured by Western blotting analysis. The results were normalized to the expression of P65 (n = 6). Data are expressed as the mean ± SD. **p < 0.01, ***p < 0.001.