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. 2021 Nov 22;12(12):1096. doi: 10.1038/s41419-021-04398-w

Fig. 5. The therapeutic effects of grafted 3D-HPMSCs on axons and functional recovery.

Fig. 5

A The in vivo process of axonal dieback at 0 day, 3 days, 7 days, and 28 days post SCI via two-photon microscopy. Some transplanted cells were observed and indicated by the white arrows at 0 days and 3 days. Asterisk indicates the lesion site. Scale bar, 100 µm. B 3D-HPMSCs group showed significantly shorter axonal dieback after SCI. C The schematic illustration of electrophysiological evaluation. D Representative recording of action potentials in mice with 3D-, 2D-HPMSCs or PBS transplantation. E 3D-HPMSCs group showed higher amplitude compared with 2D-HPMSCs and PBS group at 28 days after SCI. F 3D-HPMSCs group showed shorter latency compared to PBS group at 28 days after SCI. G SCI mice with 3D-HPMSCs transplantation achieved higher BMS score compared to 2D-HPMSCs and PBS groups. H The regularity index in 3D-HPMSCs group was significantly higher than that in PBS group at 28 d post SCI. I The base of support test suggested a better functional improvement at 28 days post SCI. ∗, ∗∗, and ∗∗∗ indicate P < 0.05, P < 0.01, and P < 0.001, respectively.