Figure 1.

B cell stimulation with Zymosan and HKCA hyphae leads to increased antibody production. Splenic mouse B cells were cultured either unstimulated or stimulated with LPS+IL-4 or anti-CD40+IL-4 in the presence of Zymosan, HKCA yeast or HKCA hyphae. (A) IgG1 class switching. Percentage of IgG1-positive cells among live B cells was measured by flow cytometry after 3 and 5 days of culture. For representative plots and gating strategy see Supplementary Figure S1C . (B) IgG1 production. The concentration of secreted IgG1 in cell culture supernatants was measured after 3 and 5 days of culture by ELISA. Data represent mean ± Standard error of the mean (SEM) of 10 mice, with triplicate measurements performed for each mouse. *p < 0.05, **p < 0.005, ***p < 0.001.