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. 2021 Nov 8;11:761346. doi: 10.3389/fonc.2021.761346

Figure 3.

Figure 3

KDM4D inhibition promotes tumor progression and chemotherapy resistance. (A) Western blot revealing the knockout efficiency of KDM4D inKYSE30 and EC109. (B) Western blot exhibiting the overexpression of KDM4D in KYSE30 and EC109. (C) Overexpression of KDM4D suppressed the colony-formation ability of ESCC cells (left panel). Quantification of the colony formation assay results (right panel). (D) CCK-8 assays showing the difference of growth rate in Ctrl and KDM4D-overexpressing cells. (E) CCK-8 assays comparing the growth differences in Ctrl and KDM4D-KO cells. (F) KDM4D deficiency promoted the migrated ability of KYSE60 and EC109 cells (left panel). Quantification of the Transwel assay results (right panel). (G) Knockout of KDM4D induced the increase in sphere numbers and sizes of ESCC (KYSE30 and EC109) cells relative to the corresponding control cells (left panel); Quantification of results from the sphere formation assays. (H) Apoptosis rates were detected in cells treated with DMSO and cisplatin. *P < 0.05, **P < 0.01, ***P < 0.001.