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. 2021 Nov 8;11:761346. doi: 10.3389/fonc.2021.761346

Figure 4.

Figure 4

Low KDM4D depends on elevated HMGB1 to promote ESCC progression. (A) Western blot assays detecting the protein levels of KDM4D and HMGB1 in KYSE30 and EC109 cells. (B) Western blot exhibiting the knockdown efficiency of KDM4D by specific shRNAs in EC109 cells (left). qPCR assay revealing the mRNA levels of HMGB1 (right). (C) CCK-8 assays revealing the cell growth differences in Ctrl and HMGB1-knockdown EC109 cells. (D–F) HMGB1 knockdown significantly suppressed the cell growth in KDM4D-deficient cells (KYSE30, EC109 and KYSE150), as indicated by the CCK-8 assays. (G) HMGB1 inhibition significantly impaired the clone formation efficiency in KDM4D-deficient KYSE30 cells. (H) HMGB1 inhibition significantly impaired the migration efficiency in KDM4D-deficient EC109 cells. (I) HMGB1 inhibition significantly impaired the self-renewal ability in KDM4D-deficient EC109 cells. (J) Representative images showing the negative associations of KDM4D and HMGB1 protein levels. Scale bar = 80μm. *P < 0.05, **P < 0.01, ***P < 0.001.