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. 2021 Nov 8;8:777452. doi: 10.3389/fmed.2021.777452

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Copyright © 2021 Tong, Yan, Wang, Yang, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Exosome characterization. (A) TEM images showed that aggregates of exosomes were distributed while connected. The exosomes had a diameter of 100–200 nm and were shaped like a double disk-like vesicular structure that was completely covered with a lipid envelope. (B) The results of exosome particle size detected by NTA showed that the median value of the overall particle size was approximately 100 nm, and particle size was mainly distributed between 50 and 200 nm. (C) Western blotting showed the positive expression of the exosome marker proteins Annexin V, CD9, Tsg101, and CD63 in the exosome group.