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. 2021 Nov 8;12:769242. doi: 10.3389/fimmu.2021.769242

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Copyright © 2021 Schäfer, Rasras, Ormenisan, Amslinger, Enzmann, Jägle and Pauly

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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C3aR expression was time-dependently regulated in FHR-3 stressed ARPE-19 cells. (A) C3AR mRNA expression was decreased after 5 h and increased after 24 h FHR-3 incubation. This effect could be confirmed on protein level: (B) Western blots of ARPE-19 cell lysates showed a tendency for decreased C3aR levels (54 kDa) 5 h after FHR-3 treatment. Supplementary Figure 1M shows full Western blots. (C) Decreased C3aR protein levels were detected by immunofluorescence using a specific anti-C3aR antibody (red) after 5 h FHR-3 incubation (upper panels), whereas no differences between FHR-3 stressed and unstressed controls were observed after 24 h (lower panels). Scale bars 40 µm. w/o untreated control (dotted line). Mean with standard deviation is shown. *p < 0.05. (A) Wilcoxon matched-pairs signed rank test (n = 3); (B) Ordinary one-way ANOVA, Turkey’s multiple comparisons test (n = 3).