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. 2021 Nov 8;12:769242. doi: 10.3389/fimmu.2021.769242

Figure 8.

Figure 8

FHR-3 induced an ARPE-19 cell dependent pro-inflammatory microenvironment. (A) NLRP3 mRNA expression in ARPE-19 cells was either increased or decreased after 5 h or 24 h FHR-3 treatment, respectively. This was in contrast to no expression changes following FHR-1, FH or FP incubation. (B) Transcripts of IL1B were significantly elevated, whereas IL18 mRNA expression was slightly decreased after FHR-3 treatment. (C, D) Apical secretion levels of (C) IL-1ß and (D) IL-18 were increased in ARPE-19 cells after FHR-3 stress. (E) FOXP3 mRNA expression was reduced 5 h and 24 h after FHR-3 incubation. (F) Secretion of IL-6 protein was significantly raised after FHR-3 incubation, both on the apical and basal cell site. (G) ARPE-19 cell secretion of TNF-α protein was on trend increased after FHR-3 incubation on the apical site compared to w/o. (H) VEGF-α was secreted by ARPE-19 cells, but showed no differences between FHR-3 treated and untreated cells. w/o untreated control (dotted line). n.d. not detected. Mean with standard deviation is shown. ***p < 0.001, **p < 0.01, *p < 0.05. (A, B, E) Wilcoxon matched-pairs signed rank test (n = 3); (C, D, F–H) Unpaired t-test (n = 5).