Figure 5.

Identification of ANGPTL4 as a target gene of miR-146a-5p. (A) Schematic illustration of the miR-146a-5p binding site in the 3′-UTR of ANGPTL4 and the matching mutant. The mutated nucleotides were marked purple. (B) The luciferase reporter plasmids (ANGPTL4-WT and ANGPTL4-Mut) were co-transfected into HEK-293T cells with miR-146a-5p mimic or its control. The luciferase activity was assessed after a duration of 48 h. (C–E) THP-1 cells were transfected with miR-146a-5p mimic/inhibitor or their negative controls for 48 h.; (C) RT-PCR analysis of miR-146a-5p expression. (D,E) The mRNA and protein levels of ANGPTL4 were detected utilizing RT-PCR and WB, in that order. Data are articulated as mean ± SD of 3 separate experiments. ^***P < 0.001.