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Validation of differentially expressed genes detected in RNA-seq with qPCR. (A) Gene expression (logFC) of DEGs used to validate RNA-seq analysis of endothelial cells incubated with B. bavariensis. Black bars—logFC values from RNA-seq, white bars—log FC calculated from qPCR. Pearson correlation coefficient (PCC) r = 0.9906; P-value < 0.01. (B) Correlation of the gene expression (log FC) of DEGs obtained from RNA-seq and qPCR after incubation of hBMECs with B. bavariesis.
