TABLE 1.
Phenotypic and genotypic characteristics of K. pneumoniae strain SH12 and its transconjugants.
| Strain ID | Bacterial species | Sequence type type |
MIC (μg/ml)
|
rmpA2 | bla KPC – 2 | Other acquired virulence and resistance genes | Conjugation efficiency | |||||||||||
| CAZ | CTX | IPM | MEM | ETP | AK | CIP | PB | ATM | TIG | CZA | FDC | |||||||
| SH12 | K. pneumoniae | 11 | >128 | >128 | >128 | >128 | >128 | >128 | 128 | 0.5 | >128 | 0.5 | 4/4 | 16 | + | + | iucA, qnrB4, blaDHA–1, sul1, msr(E), mph(E), blaTEM–1B, aac(3)-IId, blaSHV–12, mph(A), blaCTX–M–65, blaTEM–1B, fosA3, rmtB | NA |
| J53 | E. coli | NA | 0.25 | 0.25 | 0.5 | 0.03 | 0.03 | 4 | 0.008 | 0.5 | 0.125 | 0.25 | 0.125/4 | 0.25 | – | – | – | 6.00E–06 |
| J53-C1 | E. coli | NA | 16 | 32 | 0.5 | 0.03 | 0.03 | 4 | 0.008 | 0.5 | 16 | 0.25 | 0.125/4 | 2 | + | – | iucA, qnrB4, blaDHA–1, sul1, msr(E), mph(E), blaTEM–1B, aac(3)-IId, blaSHV–12, mph(A) | 2.00E–06a |
| J53-C2 | E. coli | NA | 16 | 32 | 0.5 | 0.03 | 0.03 | 4 | 0.008 | 0.5 | 16 | 0.25 | 0.125/4 | 2 | + | – | iucA, qnrB4, blaDHA–1, sul1, msr(E), mph(E), blaTEM–1B, aac(3)-IId, blaSHV–12, mph(A) | 2.00E–06a |
| J53-C3 | E. coli | NA | 16 | 32 | 4 | 4 | 4 | >128 | 0.008 | 0.5 | 16 | 0.25 | 0.125/4 | 2 | + | + | iucA, qnrB4, blaDHA–1, sul1, msr(E), mph(E), blaTEM–1B, aac(3)-IId, blaSHV–12, mph(A), blaCTX–M–65, blaTEM–1B, fosA3, rmtB | 1.00E–06 |
| J53-C4 | E. coli | NA | 16 | 32 | 0.5 | 0.03 | 0.03 | 4 | 0.008 | 0.5 | 16 | 0.25 | 0.125/4 | 2 | + | – | iucA, qnrB4, blaDHA–1, sul1, msr(E), mph(E), blaTEM–1B, aac(3)-IId, blaSHV–12, mph(A) | 2.00E–06a |
| J53-C5 | E. coli | NA | 16 | 32 | 4 | 4 | 4 | >128 | 0.008 | 0.5 | 16 | 0.25 | 0.125/4 | 0.25 | – | + | blaCTX–M–65, blaTEM–1B, fosA3, rmtB | 3.00E–06 |
| J53-C6 | E. coli | NA | 16 | 32 | 0.5 | 0.03 | 0.03 | 4 | 0.008 | 0.5 | 16 | 0.25 | 0.125/4 | 2 | + | – | iucA, qnrB4, blaDHA–1, sul1, msr(E), mph(E), blaTEM–1B, aac(3)-IId, blaSHV–12, mph(A) | 2.00E–06a |
| PM23 | K. pneumoniae | 15 | 0.5 | 0.06 | 0.5 | 0.25 | 0.25 | 2 | >128 | 0.5 | 0.25 | 0.5 | 0.5/4 | 0.25 | – | – | – | NA |
| PM23-C1 | K. pneumoniae | 15 | 64 | 128 | 0.5 | 0.25 | 0.25 | 2 | >128 | 0.5 | >128 | 0.5 | 1/4 | 16 | + | – | iucA, qnrB4, blaDHA–1, sul1, msr(E), mph(E), blaTEM–1B, aac(3)-IId, blaSHV–12, mph(A) | 1.14E–08 |
| PM23-C3-1 | K. pneumoniae | 15 | 64 | 128 | 0.5 | 0.25 | 0.25 | 2 | >128 | 0.5 | >128 | 0.5 | 1/4 | 16 | + | – | iucA, qnrB4, blaDHA–1, sul1, msr(E), mph(E), blaTEM–1B, aac(3)-IId, blaSHV–12, mph(A) | 1.48E–08 |
| PM23-C3-2 | K. pneumoniae | 15 | >128 | >128 | >128 | >128 | >128 | >128 | >128 | 0.5 | >128 | 0.5 | 1/4 | 16 | + | + | iucA, qnrB4, blaDHA–1, sul1, msr(E), mph(E), blaTEM–1B, aac(3)-IId, blaSHV–12, mph(A), blaCTX–M–65, blaTEM–1B, fosA3, rmtB | 2.96E-10 |
| 25922 | E. coli | NA | 0.25 | 0.06 | 0.25 | 0.016 | 0.03 | 4 | 0.008 | 0.5 | 0.25 | 0.25 | 0.125/4 | 0.25 | NA | NA | NA | NA |
CAZ, ceftazidime; CTX, cefotaxime; IPM, imipenem; MEM, meropenem; ETP, ertapenem; AK, amikacin; CIP, ciprofloxacin; PB, polymyxin B; ATM, aztreonam; TIG, tigecycline; CZA, ceftazidime-avibactam; FDC, cefiderocol; NA, Not available.
aConjugation efficiency for J53-C1, J53-C2, J53-C4, and J53-C6 is the same.