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. 2021 Dec 17;583:121–127. doi: 10.1016/j.bbrc.2021.10.056

Fig. 2.

Fig. 2

A. Immunofluorescence staining of neonatal rat cardiac preparations with a GFAT1-specific antibody (green) which is shown expressed in cardiomyocytes (far-red; troponin T) and fibroblasts (red; PDGFRα). Nuclei stained with DAPI (blue). Scale bar, 20 μm. B. Immunofluorescence staining of neonatal rat cardiac preparations with a GFAT2-specific antibody (green). GFAT2 is only expressed in fibroblasts (red; PDGFRα) and absent from cardiomyocytes (far-red; troponin T). Scale bar, 20 μm. C. In murine heart tissue sections, GFAT1 (green) stains both cardiomyocytes (far-red; troponin T) and fibroblast cells (red; PDGFRα). Nuclei stained with DAPI (blue). Scale bar, 20 μm. D. In murine heart tissue sections, GFAT2 only stains fibroblast cells (red; PDGFRα) and is absent from cardiomyocytes (far-red; troponin T).