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. 2021 Dec 17;583:121–127. doi: 10.1016/j.bbrc.2021.10.056

Fig. 3.

Fig. 3

A. t-distributed stochastic neighbour embedding (t-SNE) plot showing relative fold expression levels of Gfpt1 in human ventricular myocytes. Green to blue indicate 0 to 3-fold increase expression differences. B. t-SNE plot for Gfpt2 in human ventricular myocytes. C. t-SNE plot for cardiac fibroblast subpopulations showing Gfpt1 expression levels. D. t-SNE plot for cardiac fibroblast subpopulations showing enrichment in a subpopulation characterised by ILST6/Oncostatin M signalling (encircled in red). E. Western blot of adult-human iPSC-derived cardiomyocytes (CM1 and CM2 are separate differentiations and late-stage maturation) showing expression of GFAT1 but not GFAT2. Comparison with other cell types to show GFAT isoform specificity (Lane 1 – Fibro; human foreskin fibroblast cell line, Lane 2 – CM1, Lane 3 – CM2, Lane 4 – HUVEC; human umbilical vein endothelial cell, Lane 5 – U2OS; human osteosarcoma cell line. α-actinin is specific for cardiomyocytes, PDGFRα is specific for fibroblasts).