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. 2021 Nov 8;9:703836. doi: 10.3389/fcell.2021.703836

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Copyright © 2021 Díaz-Hernández, Galván-Hernández, Marín-Llera, Camargo-Sosa, Bustamante, Wischin and Chimal-Monroy.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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qRT-PCR analysis and comparison dynamics of gene expression in the presumptive ANZ and the ANZ. qRT-PCR of FGF-related genes (Fgf10, Fgfr1, Fgfr2, Mkp3, Churchill) and BMP-related genes (Bmp4, Bmp7, Bambi, Mario, Snail). (A, C, E) Comparison of gene expression between the presumptive ANZ in 22 HH vs its undifferentiated zone (UZ) and between the ANZ versus its UZ. (B, D, F) Analysis of gene expression in the ANZ at 25 HH stage relative to the presumptive ANZ at 22 HH stage (set to 1.0, red line). Data represent three independent experiments. Statistical significance was set as follows: ***p < 0.0001, **p < 0.005, *p < 0.05.