FIG. 5.

(A and B) Expression of mutant TrkA receptors in stably transfected Rat-1 cell lines. Immunoprecipitation and Western blot were performed on total cell extracts (2 mg of protein) using the 203 pan-Trk antiserum and HRP-labeled secondary anti-rabbit Ig antibody. Sizes are shown in kilodaltons. (C) [³H]thymidine incorporation by serum-starved Rat-1 cell lines stably expressing mutant TrkA receptors in the absence of NGF. Values are the means from at least five experiments performed with two independent clones of each mutant. Data are normalized to the [³H]thymidine incorporated by the cells in the presence of serum and expressed as a percentage of the incorporation measured in cells expressing the trk-5 oncogene (100%). Means and SD are shown.