TABLE 3.
Soft agar colony growth and tumor formation in nude mice caused by Rat-1 cells stably expressing mutant TrkA receptors
| Receptor | Mean no. of foci/ 103 cells ± SEM (% of control)a | No. of mice with tumors/ no. of mice tested | Latency (days) |
|---|---|---|---|
| TrkA | 0 (0) | 0/3 | |
| Trk-5 | 57 ± 4 (100) | 3/3 | 11 |
| L92V L95V | 0 (0) | 0/3 | |
| P287A | 40 ± 8 (70) | 3/3 | 11 |
| C302S | 24 ± 4 (42) | 2/3 | 24 |
| P313A | 4 ± 3 (7) | ND | ND |
| W317A | 45 ± 5 (79) | 3/3 | 18 |
| W317F | 6 ± 2 (11) | ND | ND |
| C348S | 6 ± 2 (11) | 3/3 | 32 |
a
Colonies were counted after 21 days in culture. Values are the means ± SD from at least five experiments performed with two independent clones of each mutant. Data normalized to the number of colonies formed by the trk-5-expressing clones (100%) are shown in parentheses. For tumor formation, 2 × 104 cells were injected per animal. ND, not determined.