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. 2000 Aug;20(16):5917–5929. doi: 10.1128/mcb.20.16.5917-5929.2000

FIG. 7.

FIG. 7

The ECD possesses relatively weak dimerization potential and is not required for the homodimerization of the full-length RPTPα. (A) A schematic of RPTPα constructs used in this figure. (B) Mock cross-linking and cross-linking on 293 cells transiently transfected with the construct ECD.GPI (lanes 1 to 4) or ephrin A1 (lanes 5 to 7). Shown are the results of immunoblotting analysis with MAb 12CA5 of whole-cell lysates using ECL detection. (C) In the left panel, transiently transfected 293 cells were biotinylated. Streptavidin-agarose beads were used to isolate the total biotinylated surface proteins, which were then subjected to immunoblotting analysis using MAb 12CA5 followed by 125I-labeled sheep anti-mouse IgG F(ab′)2 to determine the levels of surface-expressed RPTPα protein. In the right panel, transiently transfected 293 cells were cross-linked with BS3. Whole-cell lysates were subjected to immunoblotting analysis using MAb 12CA5 followed by 125I-sheep anti-mouse IgG F(ab′)2 to determine the levels of RPTPα dimers. n.s., nonspecific band. (D) Mock cross-linking and cross-linking on 293 cells transiently transfected with the construct ΔECD. Whole-cell lysates were subjected to immunoblotting analysis with anti-RPTPα serum 5478 using ECL detection.