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. 2021 Nov 16;2(11):100446. doi: 10.1016/j.xcrm.2021.100446

Figure 4.

Figure 4

Evaluation of cytotoxic autoimmune responses following ANGPTL3 vaccination in ob/ob mice

(A–J) Analyses of T cells and liver from ob/ob mice 6 weeks after the first immunization. (A) T cell proliferation assay of immunized mouse spleen cells performed 48 h after supplementation of culture media with vehicle, E3, KLH, or PHA (n = 5 per group). (B–E) Concentrations of (B) IFN-γ, (C) IL-2, (D) IL-4, and (E) IL-10 in culture supernatants (n = 5 per group).

(F and G) Representative images of Azan-Mallory-stained liver sections from (F) KLH and (G) E3 groups. Scale bars, 200 μm.

(H) Percentages of aniline blue-positive interstitial areas indicate degree of liver fibrosis.

(I and J) Transcript levels of (I) Col1a1 and (J) Col3a1 in liver. Transcript levels were normalized to Rps18 levels. KLH group values were set to 1.

(A, B, D, E, I, and J) Results are expressed as mean ± SEM (C) Results are expressed as median ± IQR. Veh, vehicle; n.s., not significant; ∗p < 0.05, †p < 0.01 versus control.