Figure 5.

Improved lipid profiles in severe familial hypercholesterolemia model mice following ANGPTL3 vaccination

(A–I) Blood samples from Apoe^shl mice fed a high-cholesterol diet were analyzed 6 weeks after the first immunization (n = 10 per group). (A) Titers of circulating antibodies. Values are reported as the serum dilution giving half-maximal binding (optical density: OD50%). (B) Circulating ANGPTL3 concentrations. (C–I) Levels of (C) fasting TG, (D) non-fasting TG, (E) fasting VLDL-C, (F) fasting LDL-C, (G) fasting sd-LDL-C, (H) fasting HDL-C, and (I) fasting TC in circulation.

(J and K) Evaluation of atherosclerotic plaques in aorta from Apoe^shl mice fed a high-cholesterol diet. Analysis was performed 22 weeks after the first immunization (n = 6 per group). (J) Representative photographs of Oil red O-stained aortas of indicated mice. (K) Quantitative comparison of disease severity based on atherosclerotic lesion area. (A, C, and H) Results are expressed as median ± IQR.

(B, D–G, I, and K) Results are expressed as mean ± SEM. ∗p < 0.05 and ^†p < 0.01 versus KLH (control) group.