Biological samples |
|
Newborn fecal samples |
Turku University Hospital, Turku, Finland |
N/A |
|
Critical commercial assays |
|
PureLink Microbiome DNA Purification Kit |
Invitrogen, ThermoFisher Scientific |
CAT# A29790 |
AMPure XP for PCR clean-up (magnetic beads) |
Beckman Coulter |
CAT# A63882 |
Quant-iT PicoGreen dsDNA quantitation kit |
Invitrogen, ThermoFisher Scientific |
CAT# P7589 |
MO BIO’s PowerSoil DNA Isolation Kit Handbook |
QIAGEN |
CAT# 12855 |
5x All-in-One RT MasterMix |
abm |
CAT# G592 |
Fast SYBR Green Master mix |
Applied Biosystems |
CAT# 43-856-12 |
Total RNA Purification Kit |
Norgen Biotek Corp |
CAT# 17200 |
|
Deposited data |
|
Sequence data |
This paper |
ENA:PRJEB46240; DDBJ:ERP130461 |
|
Experimental models: organisms/strains |
|
Mouse: Swiss Webster |
Azrieli Medical Faculty Animal Facility |
WT – established colony in the faculty |
|
Software and algorithms |
|
QIIME2 |
Bolyen et al., 201946
|
https://qiime2.org/ |
DADA2 |
Callahan et al., 201647
|
https://benjjneb.github.io/dada2/tutorial.html |
|
Other |
|
Mouse Chow: Maintenance Diet |
Altromin International |
Diet 1324 |