Figure 1.

Expression of miR-494-3p and key polarization markers in human macrophages treated with 3GA-494 or 3GA-ctrl

(A) Endogenous miR-494-3p expression in primary human macrophages during M1 and M2 polarization, shown as fold increase (FI) normalized to miR-494-3p expression in M0 macrophages for each donor. (B) MiR-494-3p expression in resting M0 and M1 and M2 polarized macrophages treated with 3GA-494, normalized to 3GA-ctrl treated M0, M1, and M2 macrophages, respectively. (C) M0 macrophages treated with IRDye-800-CW-labeled 3GA-494 for 24 h. Right image is a zoom-in image of the left image. Scale bars, 20 μm. Expression levels of (D) M2 markers cluster of receptors differentiation 206 (CD206), (E) interleukin-10 (IL10), (F) triggering receptor on myeloid cells 2 (TREM2), and (G) receptor for hemoglobin-haptoglobin complexes CD163 (N = 5). Expression levels in (D) to (G) were normalized to 3GA-ctrl. U6 was used as a reference gene. A one-sample t test was performed to compare single treatment with the control, within each individual donor. N is represented by the individual dots. Variations in N are caused by the exclusion criteria, as explained in materials and methods. Data are presented as mean ± SEM. ∗p < 0.05 compared with M0 (A) and 3GA-ctrl (B).