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. 2021 Nov 4;26:1228–1239. doi: 10.1016/j.omtn.2021.10.027

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Flow-cytometric analysis of human and murine polarized macrophages treated with 3GA-494 or 3GA-ctrl

Protein levels of M1 and M2 markers in human and murine in vitro polarized macrophages, treated with 3GA-494 or 3GA-ctrl for 24 h during polarization, analyzed by flow-cytometric analysis. (A) Percentage of M1 markers C-C chemokine receptor 7 (CCR7) and cluster of differentiation 86 (CD86)-positive cells in human M1 macrophages. (B) Percentage of M1 marker inducible oxide synthase (iNOS)-positive cells in murine M1 macrophages. (C) Percentage of M2 marker CD206-positive cells in human M2 macrophages. (D) Percentage of M2 marker Arginase-1 (Arg1)-positive cells in murine M2 macrophages. (A–D) Percentage (%) of positive cells, within live (A and C) CD45⁺CD11b⁺ or (B and D) CD11b⁺F4/80⁺ cells, in 3GA-ctrl- or 3GA-494-treated cells. Representative plots of both groups are shown. N is represented by the individual symbols. A two-tailed unpaired t test was performed to compare single treatment with the control. Data are presented as mean ± SEM. ∗∗p < 0.01 compared with 3GA-ctrl.