Figure 3.

mi-miR27a and si-PINK1 significantly impaired mitochondrial functions and promoted activation of activated HSCs

Activated LX2 cells were transfected with mi-miR27a and si-PINK1. (A) Parkin, p62, and LC3B protein levels were examined via western blotting. (B) Mitochondrial respiration was assessed using Seahorse. (C) α-SMA, cyclin D1, and PCNA proteins were detected in activated LX2 cells via western blotting. (D) LC3B (red IF) and COX4 (green IF) proteins and mtSOX red and mtCMXRos red were observed via IF staining. Scale bar, 25 μm. (E) Representative images and statistical analysis of cell morphology changes in PHSCs at day 7 are shown. Scale bar, 25 μm. All quantifications are presented as the mean ± SD, and p values were calculated using an unpaired Student's t test. Statistical significance: ∗p < 0.05, compared with the (B and D) mi-NC group, (E) mi-NC PHSC group at day 5 or 7; #p < 0.05, compared with the (B and D) si-NC group, (E) si-NC PHSC group at day 5 or 7.