REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Ms anti-FLAG M2	SIGMA Aldrich	Cat# F1804
Rb anti-FLAG	SIGMA Aldrich	Cat# F7425
Rb anti-Rabbit	Abcam	Cat#a b9110
	Novus	Cat# NPB2-21581
Ms anti-Myc	Invitrogen	Cat# 46-0603
Rb anti-Myc	Abcam	Cat# ab9106
	Novus	Cat# NB600-336
Ms anti-β-Actin	SIGMA Aldrich	Cat# A2228
Rb anti-KEAP1	Abcam	Cat# ab139729
Rb anti-Pink1	Novus	Cat# BC100-94
Rb anti-VDAC1	Abcam	Cat# ab15895
Ms anti-ATG5a	Abcam	Cat# Ab14748
Ms anti-GADPH	Abcam	Cat# Ab8245
Rb anti-GFP	Abcam	Cat# ab290
Rb anti-PGAM5	Atlas Antibodies	Cat# HPA036978
Secondary antibodies:
Goat anti-Mouse IR Dye 680LT	LICOR	Cat# 926-68020
Goat anti-Mouse IRDye 800CW	LICOR	Cat# 926-32210
Goat anti-Rabbit IRDye 800CW	LICOR	Cat# 926-32211
Goat anti-Rabbit IR Dye 680LT	LICOR	Cat# 926-68021
Chemicals, Peptides and Recombinant Proteins
Menadione sodium bisulfite	Sigma-Aldrich	Cat# M2518
Hydrogen peroxide solution 30%	Sigma-Aldrich	Cat# H1009
FCCP	Tocris	Cat# 0453
GSH-MEE	Sigma-Aldrich	Cat# G1404-25 MG
MG132	Tocris	Cat# 1748
DMF	Tocris	Cat# 4512
ML334	Tocris	Cat# 5625
RTA408	CAYMAN Chemical Company	Cat# 17854
RA839	Tocris	Cat# 5707
NK252	Tocris	Cat# 5293
CPUY192018	AOBIOUS, INC	Cat# AOB 9974
KI696	MedChem Express	Cat# HY-101140
TMRE	Sigma-Aldrich	Cat# 87917
MitoTracker™ Green FM	Thermo Fischer Scientific	Cat# M7514
JC10	Enzo Life Sciences	Cat# ENZ-52305
Collagen, type IV	Sigma-Aldrich	Cat# C5533
Poly-l-lysine hydrobromide	Sigma-Aldrich	Cat# P6282
RPMI Medium (IX)	Gibco, ThermoFisher	Cat# A1049101
	Scientific
DMEM, high glucose, GlutaMAX™ Supplement, pyruvate	Gibco, ThermoFisher	Cat# 31966021
	Scientific
Fetal Bovine Serum	Gibco, ThermoFisher	Cat# 10270106
	Scientific
Horse serum	Gibco, ThermoFisher	Cat# 26050088
	Scientific
B27™	Gibco, ThermoFisher	Cat# 17504044
	Scientific
B27™ Supplement minus antioxidants	Gibco, ThermoFisher	Cat# 10889038
	Scientific
RIPA Lysis and Extraction Buffer	Thermo Scientific	Cat# 89900
Intercept® Blocking buffer	Li-COR	Cat# 927-70001
IP lysis buffer (20 mM HEPES, 100 mMNaCl, 5 mM EDTA, 1% Triton X-100, 10% glycerol, pH 7.4)	Prepared in the lab
Qproteome Mitochondria Isolation Kit	Qiagen	Cat# 37612
RNAeasy mini kit	Qiagen	Cat# 74104
SuperScript III RT Kit	Invitrogen	Cat# 18080-044
FastSYBR Green Master Mix	Fermentas	Cat# K0229
	Life Sciences
RnaseZAP	Ambion	Cat# AM9780,
		Cat# AM9782
DC™ Protein Assay Reagent A, B, C	BIO-RAD	Cat# 500-0113
		Cat# 500-0114
		Cat# 500-0115
Critical Commercial Assays
ROS-Glo™ H2O2 assay	Promega	Cat# G8820
CytoTox-Glo™ Assay	Promega	Cat# G9290
Caspase-Glo® 3/7 Assay System	Promega	Cat# G8090
MitoSOX™ Red	Thermo Fischer Scientific	Cat# M36008
Experimental Models: Cell Lines
PC6-3	Gift from Dan Lindholm	RRID: CVCL_7101
HEK293	ATCC	Cat# CRL-1573
HELA	ATCC	Cat# CRM-CCL-2
NRF2 deficient mouse embryonic fibroblasts	Gift from Henrik Luuk
Oligonucleotides
rPGAM5-F-AGATGAACTGGCTTCCAGGC	TAG Copenhagen A/S
rPGAM5-R-CCTGTTCCCGACCTAATGGG
rPINK1-F-ACCTGGAGAAGGCCAAACAC	TAG Copenhagen A/S
rPINK1-R-CTGGAGGATCCTGCCGAGATA
rCycA-F-GAGCACTGGGGAGAAAGGAT	TAG Copenhagen A/S
rCycA-R-CTTGCCATCCAGCCACTCAG
rKEAP1-F-TCTTCAACCTGTCACACTGC	TAG Copenhagen A/S
rKEAP1-r-ATCTCACACTTCTGCAGCTG
Recombinant DNA
KEAP1 shRNA	SABiosciences	Cat# KR47610 N
AAGGTCATGGAGAGGCTAATT (no 2)
GGACTACCTGGTGCAGATCTT (no 3)
PGAM5 shRNA	OriGene	Cat# TR702898 (TI711593)
ATTGAGGCTGCCTTCAGGAACTACATCCA
PINK1 shRNA	SABiosciences	Cat# KR55105 N
CTGCAATGCCGCTGTGTATGA
DRP1 shRNA	SABiosciences	Cat# KR45077 N
CGAGCAGGGTGTTCTGAACTT
FUNDC1 shRNA	Origene	Cat# TR702819
Mito-CFP	Clontech.	Cat# 632432
DsRed2-Mito	Clontech.	Cat# 632421
mKate2-mito	Evrogen	Cat# FP187
KillerRed-dMito	Evrogen	Cat# FP964
MLS-HyPer7	Addgene	Cat# 136470
hSyn-EGFP	Addgene	Cat# 50465
EGFP-LC3B	Addgene	Cat# 24920
		Cat# 11546
		Cat# 24920
GFP-LC3C	MRC reagents	Cat# DU40779
Parkin-EYFP and Parkin-EYFP C431 N	Gift from Richard Youle
Parkin-EYFP T340R	generated from Parkin-EYFP by Mutagenex Inc.
PINK1-EYFP	Addgene	Cat# 101874
PINK1	Gift from Emma Deas
HSP90AA1	SinoBiological	Cat# HG11445-UT
Myc-RICTOR	Addgene	Cat#11367
MAPKAP1-Myc	Addgene	Cat#12576
APPL2	Harvard PlasmID Database	CloneID: HsCD00335054
GRP75	Gift from Gyorgy Szabadkai
Flag-NRF2 WT and Flag-NRF2 ETGE to GAGA	Generated in our lab
FLAG-KEAP1 and FLAG-KEAP1 R380A-R415A	Generated in our lab
HA2-KEAP1	Addgene	Cat# 21556
PGAM5-Myc-DDK	Origene	Cat# RC229840
PGAM5-Myc, PGAM5-Myc E79A/S80A, PGAM5-Myc F244D, PGAM5-Myc S24F	Generated from the previous plasmid by EUROFINS
PGAM5-HA	MRC reagents #DU46867
PGAM5-YPet	Synthesized by Thermo Fischer
PGAM5-Myc-DDK transcript variant 3 (isoform 2, UniProt #Q96HS1-2)	OriGene	Cat# RC201678
TOMM7- Myc-DDK	Origene	Cat# RC210040
PARL-FLAG	Addgene	Cat# 13639
OMA1-FLAG	Addgene	Cat# 61719
HyPer7	Addgene	Cat# 136466
MLS-HyPer7	Addgene	Cat# 136470