FIGURE 3.

Cell proliferation and Pathway Inhibition Assay. (A) Effect of selected inhibitors of Akt, mTOR, STAT, and JAK on the KAIMRC1 cell line. Akt inhibitor API‐2 showed significant growth inhibition in contrast to other inhibitors, suggesting that the Akt pathway is crucial for the survival of KAIMRC1 cells. (B) Effect of JAK2 inhibitor, ruxolitinib on KAIMRC1, MDA MB‐231, and MCF‐7 cell lines. CellTiter‐Glo® assay was performed to assess cell viability after compound treatment. The cells were treated with compounds in complete media with serum 24 h prior to the assay. (C) Comparison of ruxolitinib IC₅₀ of three cell lines. KAIMRC1 cell line showed lowest IC₅₀ in comparison to MDA MB‐231 and MCF‐7 cell lines, suggesting that the inhibition of the JAK2 pathway can induce cell cycle inhibition and apoptosis in KAIMRC1 cells. x‐axis = Normalized Relative light unit (RLU) and y‐axis = Logarithm of the drug concentration in molarity (Log[drug]M)