Skip to main content
. 2021 Nov 10;65(Suppl 1):3285. doi: 10.4081/ejh.2021.3285

Figure 2.

Figure 2.

Oxaliplatin-induced subcellular impairment and its protective effects of micronutrients. A) The ROS were evaluated in order to highlight the role of oxaliplatin to induce oxidative stress; microglial BV-2 cells increased the ROS production during oxaliplatin treatment; such an increase in ROS overproduction was counteracted by the presence of micronutrients in the cell medium; values are expressed in percentage of control (Ctrl, untreated cells) as mean ±SEM; “*p<0.05 vs Ctrl; #p<0.05 vs oxaliplatin. Each experiment was performed in triplicate, for three different experimental setups. B) Immunofluorescent analysis of cytochrome C shown a significant cytoplasmic increase after 24 h of 3 μM oxaliplatin treatment (oxaliplatin); on the other hand, pre- and co-treatment of Mg 1 mM, Mn 50 nM and Zn 100 nM 1 mM Mg, 50 nM Mn and 100 nM Zn (grey columns and last three images on the right) were able to prevent this incremen. Five microscopic fields for each experimental point were analyzed, and three different experiments were performed; blue, DAPI; green, Cytochrome C. Total magnification 200x; scale bar: 50 μm. Values are expressed in percentage of control (Ctrl, untreated cells) as mean ±SEM; *p<0.05 vs Ctrl; #p<0.05 vs oxaliplatin. C) The Nrf2 analysis revealed a consistent nuclear translocation when BV-2 cells were treated with 3 μM oxaliplatin alone for 24 h; on the contrary, when Mg, Mn and Zn were added in the medium, they counteract the translocation of the Nrf2 transcription factor which showed mainly a cytoplasmic localization. Five microscopic fields for each experimental point were analyzed, and three different experiments were performed; red, DAPI; green, Nrf2. Total magnification 200x; scale bar: 50 μm. Values are expressed in percentage of control (Ctrl, untreated cells) as mean ±SEM; *p<0.05 vs Ctrl; #p<0.05 vs oxaliplatin. D) Western blotting analysis and quantification of GRP78 expression during oxaliplatin 3 μM treatment at 24 h alone (black column) and in presence of 1 mM Mg (light grey column), 50 nM Mn (medium grey column) or 100 nM Zn (dark grey column), both in pre-treatment and co-treatment; values are expressed in percentage of control (Ctrl, untreated cells) as mean ±SEM; *p<0.05 vs Ctrl; #p<0.05 vs oxaliplatin. Each experiment was performed in triplicate, for three different experimental setups.