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. 2021 Nov 12;23(1):16. doi: 10.3892/ol.2021.13134

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Copyright: © Ma et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — MFC cell apoptosis induced by luteolin (20 µM) and/or oxaliplatin (5 µM). (A) The morphological changes indicative of MFC cell apoptosis were assessed by fluorescence inverted microscopy (magnification, ×200). (B and C) After double-staining with Annexin-V FITC and PI, flow cytometry was used for the quantitative analysis of MFC cell apoptosis. (D) Protein expression levels (Bcl-2, Bax, and β-actin) were assessed by western blot analysis. (E) Scanning gray analysis was calculated by Photoshop CC software (Adobe Systems Inc.). Data are presented as mean ± SD, **P<0.01. Experiments were repeated at least 3 times. MFC, mouse forestomach carcinoma; Lut, luteolin; Oxa, oxaliplatin; Bax, BCL-2-associated X protein; Bcl-2, B-cell lymphoma 2.