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. 2021 Nov 12;23(1):16. doi: 10.3892/ol.2021.13134

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Copyright: © Ma et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — ROS accumulation and mitochondrial membrane potential in mouse forestomach carcinoma cells induced by luteolin (20 µM) and/or oxaliplatin (5 µM). (A) Fluorescence intensity of DCFH-DA was captured under a fluorescence inverted microscope system (magnification, ×200). (B and C) ROS levels were analyzed using DCFH-DA staining and flow cytometry. (D and E) Mitochondrial membrane potential was analyzed by JC-1 staining and flow cytometry. *P<0.05, **P<0.01. Experiments were repeated at least in triplicate. Data are presented as mean ± SD. Lut, luteolin; Oxa, oxaliplatin; ROS, reactive oxygen species.