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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: FASEB J. 2021 Oct;35(10):e21940. doi: 10.1096/fj.202100944R

Figure 4. BHLHE40 deficiency dampens pro-inflammatory gene expression.

Figure 4.

(A-D) Lyz2cre/cre and Bhlhe40fl/fl:Lyz2cre/cre mice PMs were stimulated with 100 ng/ml LPS for 4 hours, and RNAseq data were subjected to GSEA studies. The LPS challenge significantly elevated gene targets involved in allograft rejection (A), IFNγ and INFα response (B), inflammatory and IL6-JAK-STAT3 response (C), and complement response (D) in Lyz2cre/cre mice macrophages. These inflammatory signaling responses were significantly dampened in Bhlhe40fl/fl:Lyz2cre/cre mice macrophages (n=3). Heat maps for allograft rejection, IFNγ/IFNα response, and inflammatory response represent the top fifty differentially expressed genes in the presence and absence of LPS. GSEA results from IFNγ and IFNα response data set were combined to generate panel B. Similarly, Inflammatory and IL6-JAK-STAT3 were combined to generate panel C.