Skip to main content
. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: FASEB J. 2021 Oct;35(10):e21940. doi: 10.1096/fj.202100944R

Figure 5. Impact of BHLHE40 deficiency on LPS-induced pro-inflammatory gene expression.

Figure 5.

(A-D) Lyz2cre/cre and Bhlhe40fl/fl:Lyz2cre/cre mice PMs were stimulated with 100 ng/ml of LPS for 4 hours. Total RNA samples were analyzed for expression of (A) allograft rejection (Nos2, Ifnγ, Il12a, Tnf, Nlrp3, Cxcl9), (B) IFNα/IFNγ response (Cd38, Ptgs2, Fpr1, Socs3, Cd274, Irf8), (C) inflammatory and IL6-JAK-STAT3 response (Cd14, Ccr7, Il6, Slc7a2, Itga4, Ccl7), and (D) complement (Mmp14, Kynu, C3, Mmp13, Serpinb2, Cfb, Il1β, Icam1) target genes were analyzed by RT-qPCR. 36B4 was used as a housekeeping gene (n=3). Values are reported as mean ± SD. Data were analyzed by Student’s t-test. *p < 0.05, ** p < 0.01 and ***p < 0.001.