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. 2021 Oct 10;2:31. doi: 10.1186/s43556-021-00053-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Cldn expression in human prostate cells and tissue specimens. a The levels of Cldn3 and Cldn4 were higher in metastatic human prostate cancer (PC3, LNCaP, and DU145) cells compared to benign prostate (RWPE-1) cells. Immunohistochemistry was performed on (b) human benign prostatic hyperplasia specimen, (c) human high-grade prostate cancer specimen 1 (Gleason grade group 4 +), and (d) human high-grade prostate cancer specimen 2 (Gleason grade group 4 +) for Cldn4 (brown). Representative H&E and IHC staining are shown at 100× and 400×. Scale bars = 50 μM