Fig. 2.

Knock down of Cldn3 and Cldn4 in human prostate cancer cells. Western blots showing the effects of Cldn3 siRNAs (siCldn3) and Cldn4 siRNAs (siCldn4) on the expression of (a) Cldn3 and (b) Cldn4 respectively, in human PC3 and LNCaP prostate cancer cells after 72 h. Four Cldn3 and Cldn4 siRNAs were tested. c A heatmap demonstrates the percent knockdown (i.e., Color scale is used where white represents a 100% knockdown of protein expression and dark blue represents a 0% knockdown of protein expression) for each siRNA target sequence for PC3 and LNCaP prostate cancer cells. d Relative Cldn3 protein levels in LNCaP prostate cancer cells after 2 weeks of siCldn3 and siCldn4 treatment