Fig. 3.

V158411 increases cytoplasmic dsDNA in cancer cell lines. a Cells were treated with 3x γH2AX EC₅₀ V158411 (2.4, 1.9 and 0.78 μM for HT29, U2OS and HCC1937 respectively) for 24 h and cytoplasmic dsDNA determined by high content imaging with two different mouse monoclonal antibodies. b Nuclei staining positive for dsDNA were determined from (a and b adapted from [23]). Open and filled symbols indicate data from the two different mAbs. Mean of 3 independent wells ± SD