Fig. 5.

Stochastic simulations of the wild type model. MaBoSS was used to simulate the evolution of cell populations for each of the considered environmental conditions, starting from naive Th0 state. The probabilities associated with each phenotype is computed from the number of stochastic simulations leading to each phenotype from pre-defined initial conditions. a Wild type simulation in the presence of pro-Th1 cytokines, which gives rise to IFN-γ⁺ cells. b Wild type simulation in the presence of pro-Th17 cytokines, which gives rise predominantly to IL-17F+⁺ cells, a smaller proportion of double IL-17A +⁺ IL-17F+⁺, and a still smaller proportion of single IL-17A+⁺ cells. c Wild type simulation in the presence of IL-12 + IL-1β, which give rise to cells expressing combination of IL-2, IFN-γ and/or IL-17F (but not IL-17A). In each case, the large grey sector correspond to cells remaining in a non activated state. Although the percentages obtained are sensitive to updating rates, these simulations point to key differences in the specific cell phenotypes obtained for different combinations of Th1/Th17 polarizing cytokines