Fig. 3.
The CMV-miR-221/222 sponge combined miR-221 and miR-222 to de-repress ERα and PTEN expression in MCF-7TamR cells. a Schematic of the miR-221/222 sponge expression cassette. I: bulged/imperfect complementarity towards miR-221; II: bulged/imperfect complementarity towards miR-222. b The expression of miR-221 and miR-222 was analyzed by qRT-PCR 48 h after the transfection of the CMV-miR-221/222 sponge. Simultaneously, a biotin-labelled miR-221/222 sponge probe was used to pulldown miR-221 and miR-222. Then the amount of miR-221 and miR-222 was assessed by qRT-PCR (c). In addition, qRT-PCR and western blotting were used to explore the expression of ERα and PTEN after the transfection of the miR-221/222 sponge expressing vector in MCF-7TamR cells (d, e). Data in b-e were presented as the mean ± SD (n = 3). ***p < 0.001