Fig. 4.
T-VISA -miR-221/222 sponge inhibited cell growth of MCF-7TamR cells in a 3D cell-culture model. a hTERT expression profile across all tumor samples and paired normal tissues. Each dot represents the expression in one sample. The red dots represent tumor samples, the green dots represent normal samples. T: Tumor, N: Normal. BRCA: Breast cancer. b Schematic representation of the hTERT-based promoter-driven miR-221/222 sponge construct (T-VISA-miR-221/222 sponge). c The mRNA expression of the miR-221/222 sponge was analyzed by qRT-PCR after 48 hours transfection with the T-VISA-miR-221/222 sponge expressing vector. d The protein level of ERα and PTEN in MCF-7TamR cells was determined by western blotting after 72 hours transfection with the T-VISA-miR-221/222 sponge expressing vector. e The effect of the T-VISA-miR-221/222 sponge on cell growth of the GFP-expressing reporter in MCF-7TamR 3D cell culture. The data in c-e were presented as the mean ± SD (n = 3). **p < 0.01, ***p < 0.001