Fig. 2.

Introducing random sequences into mammalian genomes by Random-PE. a. Diagram showing the design of pegRNAs targeting Actin-b, VEGFA and DNMT1 loci. PBS was shown in green; HA was shown in cyan and the random sequence was shown in red. b. Targeted insertions of 5 bp, 8 bp and 10 bp random sequences in Actin-b, VEGFA and DNMT1 loci (PBS = 13 nt, HA = 10 nt). c. Optimization of the HA length in different editing events. Upper panels showed the efficiencies of targeted insertions of 5 bp, 8 bp and 10 bp random sequences in Actin-b, VEGFA, and DNMT1 loci at indicated HA lengths, and lower panels showed the level of undesired indels accompanied with the insertions. Values and error bars reflect mean ± s .d. of n = 3 independent biological replicates