Fig. 2.

Compensatory signaling supported the survival of melanoma cells with MAPK and PI3K dual inhibition. a Dose-dependent suppression of MAPK and PI3K pathways by AZD6244 (0, 0.1, 1, 10 μM) combined with BEZ235 (0, 0.01, 0.1, 1 μM) in WM2664 and SKMEL28 parental and DPRs (left). Phosphorylation levels of these proteins in parental or DPRs treated with AZD6244 (1 μM) and BEZ235 (0.1 μM) for indicated durations (h) (right). b IHC analysis of indicated proteins in tumors grown with or without treatment of AZD6244 and BEZ235 (scale bar = 100 μm). Image is representative of five independent experiments